Anatomical Pathology Laboratories
For tissue review and immunohistochemistry for amyloid typing
Specimens can be sent to a reference laboratory for assistance or second opinions on Congo red staining or immunohistochemistry. Each laboratory has specific requirements as outlined below. Please contact the laboratory for further information as required.
Use the map at left
to view clinics in applicable states.
New South Wales
Address
Anatomical Pathologist
Department of Anatomical Pathology
Level 3 ICPMR
Westmead Hospital
Hawkesbury Rd
Westmead NSW 2145
Phone 02 8890 6222
Fax 02 9687 2330
Email NSWPATH-Westmead-ICPMRAPOffice@health.nsw.gov.au
Sample requirements
Tissue block and Congo Red stained slide
If the referring lab cannot release tissue blocks, send the following unstained slides:
2x 4 micron thick unstained slide
8x 7micron thick unstained slides
(Please label which slides are which)
Document requirements
A copy of the original pathology report
Request for amyloid protein typing using immunohistochemical (IHC) stains
Test specification
Review of Congo Red stained slide and examination under polarised light to confirm the presence of amyloid
Amyloid protein staining using TTR, kappa, lambda and AA IHC stains
Cost Medicare rebated if both a pathologist and clinician request review
Turnaround time
3-5 business days
Victoria and Tasmania
Address
Dr Patrick Hosking
Senior Pathologist, VTAS
Level 5, Building B
Box Hill Hospital
8 Arnold Street
Box Hill VIC 3128
Phone: 03 9895 3477
Fax: 03 9895 3184
Note: these numbers are not for patients or general public enquiries
Sample requirements
The original H&E and Congo red stained slides plus 8 unstained slides of routine thickness for immunohistochemistry.
Document requirements
Please include:
- A copy of the original pathology report
- The pathologist’s name and provider number requesting/consenting to the second opinion and/or additional immunohistochemistry
- The pathologists’s authorised signature as per the request form.
- The downloaded request form is applicable when the treating clinician and the pathologist (who provided the original opinion) agree upon a second opinion. A Medicare rebate is provided in this circumstance.
Test specification
Review of tissue by Congo Red Staining and examination under polarised light for the presence of amyloid
A standard panel of kappa and lambda IHC and IF, amyloid A and transthyretin IHC is performed, and assistance with referral for further testing for rare amyloid types including mass spectrometry.
Cost Medicare rebated if both a pathologist and clinician request review
Turnaround time
3-5 business days
Queensland
Address
Department of Anatomical Pathology
Princess Alexandra Hospital
199 Ipswich Rd, Woolloongabba QLD 4102
Email: Amyloidosis@health.qld.gov.au
for “Request for amyloid tissue review” along with original report, clinical information and the reason for review.
Further instructions will be provided and tailored testing will be performed for each request.
Western Australia
Address
Dr Ian Sinclair
Dept of Anatomical Pathology
Fiona Stanley Hospital
Murdoch Perth 6150
Phone: 08 6152 8449
Fax: 08 61528052
Note: these numbers are not for patients or general public enquiries
Sample requirements
Tissue block and all stained slides
If the referring lab cannot release tissue blocks, please send the following unstained slides:
1x 4micron unstained section (for H&E)
7x4micron unstained sections on charged slides for immunohistochemistry (includes two spares)
1x7micron unstained section (for Congo red)
(Please label which slides are which)
Test specification
Examination of tissue sections with H&E and Congo red to identify and localise amyloid
Immunohistochemistry as required (amyloid A, amyloid P, TTR, kappa, lambda)
Cost Medicare rebated if both a pathologist and clinician request review
Turnaround time
3-5 business days
