Mass Spectrometry

Light dissection and mass spectrometry of amyloid deposits from paraffin embedded tissue block

Mass Spectometry is the current gold standard method for subtyping amyloidosis deposits. This is particularly necessary if a patient has both features of AL and TTR amyloidosis or in rarer cases of amyloidosis, such as isolated renal disease. For example, if a patient phenotypically has TTR amyloidosis but has a paraprotein or light chain excess appropriate subtyping is required.

Accurate subtyping is necessary to ensure the patient is managed with the correct treatments and minimises morbidity and mortality from inappropriate treatments and uncontrolled amyloidosis.

Mayo Clinic Laboratory, Rochester, USA

SydPath and the Mayo Clinical Laboratories, Rochester US, have established a reference laboratory testing collaboration and send away service.

SydPath manage the end-to-end testing service from Australia to the US, which includes sample retrieval, handling, expedited transport, and reporting for Amyloid Mass Spectrometry.

Prepayment options include credit card or via an invoice for approved institutions (with a valid PO).

Contact

Michelle Blackwell, SydPath Interim Operations Manager, michelle.blackwell@svha.org.au

Send samples to

Attn: Michelle Blackwell
SydPath Interim Operations Manager
Level 6, Xavier Building St Vincent’s Hospital Sydney
390 Victoria St Darlinghurst 2010

Sample requirements

Formalin fixed tissue block and Congo Red Slides where available.

Test Name

AMPIP- Amyloid Protein Identification, Paraffin, LC-MS/MS

Test specification

Laser dissection of amyloid deposits followed by tandem mass spectrometry analysis of the constituent amyloid protein

Send

Completed request form (standard pathology request form is acceptable – download below) along with a clinical letter outlining patient history and relevant background information, please include physician’s email for return of results.

Cost

POA, ~$2000 AUD per sample, please contact Michelle at SydPath – each request will be provided a quote based on the service required. Note: prices are subject to variation due to exchange rates, shipping, and handling/domestic transport. To ensure compliance with NPAAC sample storage requirements, the specimen blocks/slides are returned by the Mayo Clinic Laboratories via SydPath after testing is complete.

TAT

14-21 days – results are sent via encrypted email as soon as they are reported by the Mayo Clinic Laboratories.

Queensland Amyloidosis Centre, Pathology Queensland

Contact

amyloidsubtyping@health.qld.gov.au

Ph: 07 3176 2111

Address:
Central Specimens Reception
Pathology Queensland
Princess Alexandra Hospital
Building 1, Level 1, 199 Ipswich Road
Woolloongabba

Test Name

Amyloid Mass Spectrometry Request form

Sample Requirements

Formalin fixed tissue block, congo red stained slide

Send

Request form with the existing pathology report

Cost

$1538.39

TAT

1 month

UK NAC, Royal Free Hospital London

Contact

rf-tr.nachistology@nhs.net

Histology enquiries +44 20 7433 2753

Address:
Specimen Reception
Jack O’Neill Laboratory (JONAL) National Amyloidosis Centre
Royal Free Hospital,
London NHS Foundation Trust,
Pond St London NW3 2QG, UK

Test name

Amyloid histology, immunohistochemistry and proteomic analysis

Sample requirements

Formalin fixed tissue block at room temperature

OR

22 serial sections, one section per slide, on coated slides or equivalent, dried overnight at 37°C,
CUT as follows:

  1. Section # 1 6mm thick
  2. Sections # 2 – 13, – 2mm thick
  3. Sections # 14 -21, 6mm thick
  4. Section #22 – 6mm thick, on the same slide as section #1 if possible.
  5. Please put sections in the middle of the slide.

OR

If only small amount of material is available – 9x 6mm thick sections

NOTE: Due to UK custom regulations the UK NAC have difficulty in returning stained slides and FFPE blocks; if you send the block for review at the NAC please provide permission to store/keep the samples in the archive file to avoid samples being lost or destroyed.

Send

Request form including payment details, Cover letter with all clinical information available, existing histology report

Cost

£1500

TAT

3 months

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